Journal: eLife
Article Title: Peptidoglycan recycling is critical for cell division, cell wall integrity, and β-lactam resistance in Caulobacter crescentus
doi: 10.7554/eLife.109465
Figure Lengend Snippet: ( A ) Phase contrast images of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells, harvested in the exponential and stationary growth phase. Scale bar: 5 µm. ( B ) Superplots showing the distribution of cell lengths in populations of C. crescentus wild-type, Δ anmK (PR252), and Δ amgK (PR262) cells in the exponential (exp) and stationary (stat) growth phase. Data (n=100 cells per replicate) are presented as described for . The statistical significance ( p -value) of differences between conditions was assessed using a two-sided Welch’s t -test. ns indicates p -values >0.1. ( C ) Serial dilution spot assay investigating the growth of C. crescentus Δ anmK (PR252) and Δ amgK (PR262) on plates containing different concentrations of ampicillin. The cells were spotted on the same plates as those depicted in . See for all replicates (n=3 independent experiments). ( D ) Levels of N-acetylmuramic acid (Mur N Ac) in the cytoplasm of C. crescentus wild-type and Δ amgK (PR262) cells, measured by metabolomics analysis through quantification of the corresponding mass spectrometric peak areas. The mass spectrometric peak areas were normalized against the mean obtained for the Δ amgK mutant. The statistical significance of differences between the two strains was determined using a two-sided Welch’s t -test. ( E ) Analysis of the growth of C. crescentus wild-type, Δ nagK (PR255), and Δ amgK (PR262) cells on agar containing a fosfomycin gradient. The fosfomycin concentrations on the MIC test strip are indicated in the legend on the right.
Article Snippet: Chemical compound, drug , fosfomycin MIC test stripes , Liofilchem, Italy , Cat. #: 92078 , .
Techniques: Serial Dilution, Spot Test, Mutagenesis, Stripping Membranes
